Journal:

Article Title: Participation of the interstitium in acute immune-complex nephritis: interstitial antigen accumulation, cellular infiltrate, and MHC class II expression

doi: 10.1046/j.1365-2249.2003.02188.x

Figure Lengend Snippet: Cellular infiltration, proliferation and expression of MHC class II molecules

Article Snippet: To determine the percentage of MHC positive cells expressing CD11b, tissues were stained with anti-MHC class II molecules and for CD11b by immunofluorescence double staining technique as described before [ 14 ] and using the following secondary antibodies: FITC, affinity-purified F(ab′) 2 rat antimouse IgG adsorbed with rabbit serum (Accurate Chemical and Scientific Corp.) at a concentration of 60 g/ml for anti MHC class II antibody and rhodamine-conjugated rat antimouse IgG with adsorbed with rabbit serum (TRIC; Accurate Chemical and Scientific Corp.) at a concentration of 20 mg/ml for CD11b.

Techniques: Expressing

Journal:

Article Title: Trypanosoma cruzi mitochondrial malate dehydrogenase triggers polyclonal B-cell activation

doi: 10.1046/j.1365-2249.2002.01746.x

Figure Lengend Snippet: (a) p33 up-regulates expression of B-cell activation markers. B cells were cultured in the presence of macrophages with p33 (20 μg/ml) or medium alone for 3 days. The cells were then stained with PE-labelled antimouse CD19+ and FITC-labelled antimouse B7.1, B7.2 or FITC-labelled antimouse CD19 and PE-labelled antimouse MHC class II molecules. CD19+ cells were gated and analysed for the expression of surface molecules mentioned above. We show on p33 stimulated B cells (grey fill histogram): (i) expression of B7.2 + B cells; (ii) expression of MHC class II + B cells; (iii) expression of B7.1 + B cells. In all figures thick black line histograms represent B cells cultured without stimulus, thin grey line histograms represent unstained cells. This set of results is representative of three individual experiments. (b) p33 effect on immunoglobulin secretion. B cells in the presence of macrophages (3 × 106/well) were incubated with medium alone (□) or with 20 μg/ml p33for 6 days. Cell free supernatants were harvested and analysed for Ig isotype production by ELISA. Data are presented as represent the mean ± s.d. The asterisks indicate significant differences (*P < 0·05,**P < 0·01) compared with unstimulated B-cells.

Article Snippet: After this time, the cells were then stained with PE-labelled antimouse CD19 + and FITC-labelled antimouse B7.1, B7.2 or FITC-labelled antimouse CD19 and PE-labelled antimouse MHC class II molecules (PharMingen, San Diego) antibodies for 30 min at 4°C.

Techniques: Expressing, Activation Assay, Cell Culture, Staining, Incubation, Enzyme-linked Immunosorbent Assay